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KMID : 0366319900100030170
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Biochemistry and Molecular Biology News
1990 Volume.10 No. 3 p.170 ~ p.173
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The Use of Recombinant DNA Techniqes to Study Sterol Metabolism in Streptomyces sp.
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Paik Soon-Young
Sawada Shozo
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Abstract
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The nucleotide sequence of the cholesterol oxidase gene (choA) of Streptomyces sp. was determined previously. We searched for the promoter region of the choA gene and found a strong promoter at about 1.3-kb upstream from choA gene. Thus we sequenced the fragment between the promoter and choA gene and found a new open reading frame (ORF), unexpectedly. Furthermore, the results of transcript analysis suggested that the choA gene was transcribed polycistronically under the control of the promoter. In this review, we also discuss homologies in the new ORF and its activity of cytochrome P-450.
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KEYWORD
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